elyra 7 microscope for specialised 3d structured illumination (sim2)  (Carl Zeiss)

Journal: Nature Communications

Article Title: A hollow TFG condensate spatially compartmentalizes the early secretory pathway

doi: 10.1038/s41467-025-59118-1

Figure Lengend Snippet: a Representative widefield micrograph of HeLa cells with homozygous endogenously tagged TFG::mClover-FLAG. Scale bar 10 μm. Magnified micrograph (dashed box in cell overview): individual TFG condensate. Scale bar 500 nm. b Quantification of the diameter of endogenous TFG condensates = 30. The dashed line represents the average of in vitro condensate diameter (341 nm ± 87) and the dotted line represents the average of endogenous ER/Golgi interface diameter (269 nm), for reference. c Qualitative fluorescence recovery after photobleaching (FRAP) of a TFG condensate. Scale bar 500 nm. d Left: live-cell confocal microscopy of large individual condensates within HeLa cells transfected with TFG-mEGFP-FLAG. Scale bar 5 μm. Middle: STED micrograph of large individual condensates within HeLa cells transfected with TFG-mEGFP-FLAG. Scale bar 2 μm. Right: 3D-structured illumination microscopy (3D-SIM) of large individual condensates within HeLa cells transfected with TFG-tGFP. Scale bar 500 nm. e Representative micrographs of partial and full FRAP experiments on overexpressed TFG condensates in HeLa cells. f Quantification of average condensate recovery rates for partial ( n = 33) and full ( n = 30) FRAP; normalized to pre-bleach (=100) and post-bleach intensity (=0). Error bars represent standard deviation. g Representative live-cell imaging of TFG condensate fusion dynamics. h Line scan of condensate depicted in ( g ). Source data are provided as a Source Data file.

Article Snippet: Images of sponge-like TFG condensates (Supplementary Fig. ) were obtained via 3D structured illumination microscopy (3D-SIM), and images were reconstructed employing OMX-specific software packages (softWoRx).

Techniques: In Vitro, Fluorescence, Confocal Microscopy, Transfection, Microscopy, Standard Deviation, Live Cell Imaging

Journal: Cell reports

Article Title: Identification of protein aggregates in the aging vertebrate brain with prion-like and phase-separation properties

doi: 10.1016/j.celrep.2023.112787

Figure Lengend Snippet:

Article Snippet: 3D Structured Illumination microscopy , Applied Precision-GE , DeltaVision OMX BLAZE system.

Techniques: Virus, Recombinant, Protease Inhibitor, Staining, Capsules, Sterility, Plasmid Preparation, Bicinchoninic Acid Protein Assay, Mutagenesis, Mass Spectrometry, Cloning, Sequencing, Microscopy, Membrane, Expressing, Software